ACTION OF DRUGS ON THE RABBIT'S EYE

EXPERIMENT

Requirements

Animals Required: Rabbits

Apparatus Required: Droppers, measuring scale, torch, cotton wool, calculator (optional).

Drugs & solutions:

1. Saline

2. Eserine salicylate 0.5%

3. Atropine sulphate 1.0%

4. Phenylephrine 2.0%

5. Lignocaine 1.0%

PROCEDURE

· Place the rabbit (No.1) on the table.

· Measure the diameter of both the pupils with the help of a scale.

· Observe the condition of the conjunctiva (congested or not) and elicit the corneal and light reflexes.

· Record your findings.

· To the left Eye put one drop of saline and in the right eye one drop of eserine Salicylate.

· Use the pouch method for instilling the drops.

· After adding the drops, the medial canthus should be pressed for 10 seconds.

· Record the following parameters at one minute, 5minutes and ten minutes after instilling the drug and saline.

· Parameters to be measured:

1. Diameter of the pupil

2. Light reflex

3. Corneal reflex

Record your observations in a tabular form. Repeat the same procedure for atropine, phenylephrine and lignocaine on separate rabbits (Nos 2,3 & 4).

Presentation of data and analyses:

· Pool the data from other groups and formulate appropriate table(s) to display the data.

· Analyse the data using appropriate statistical test(s) and draw conclusions.

· Make sure that table(s) is/are complete in all respects.

RESULT: The action of Drugs on rabbit’s Eye was studied.